微波辅助酶催化甜菊苷的转苷和水解

Microwave-Assisted Enzymatic Transglycosylation and Hydrolysis of Stevioside

DOI：10.3969/j.issn.1673-1689.2015.12.017

中文关键词: 微波 甜菊苷 转苷 水解

英文关键词: microwave,stevioside,transglycosylation,hydrolysis

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中文摘要:

分别研究了微波辅助α-环糊精转移酶（α-CGTase）催化甜菊苷（St）转苷，和β-半乳糖苷酶催化St水解的反应。实验表明，微波能够加速来源于Paenibacillus macerans JFB05-01的α-CGTase催化St转苷反应，催化效率提高了21.7倍；但微波对β-半乳糖苷酶催化St的水解反应的影响较小。α-CGTase的加酶量为1 000 U/g时，反应1 min，St的转化率高达71.6%，St-Glc 1的产率为23.7%。

英文摘要:

Microwave-assisted enzymatic transglycosylation and hydrolysis of stevioside （St） were investigated using α-cyclodextrin glucanotransferase （α-CGTase） and β-galactosidase，respectively. Microwave irradiation could accelerate the transglycosylation of St using α-CGTase extracted from Paenibacillus macerans JFB05-01，and the catalytic efficiency was increased by 21.7 folds. However，little effect was observed when microwave irradiation was applied to the enzymatic hydrolysis of St using β-galactosidase. With 1 000 U/g of α-CGTase was loaded，the conversion of St could reach as high as 71.6% after 1 min and the yield of St-Glc 1 was 23.7% correspondingly.

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